An immune repertoire is the sum total of functionally diverse T and B cells in a body at any given moment. It is both a snapshot and historical record of a person’s immune function. Sequencing the expressed T- and B-cell receptor genes in the immune repertoire allows for an accurate evaluation of a person’s health status and risks for different diseases.
We offer reagents and services to amplify and sequence the immune repertoire with Next Generation Sequencing (NGS). Our primer kits are compatible with both Illumina and Roche 454 sequencing platforms. Read more to discover the best options for meeting your research goals.
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iRepertoire has developed a novel, patented mulitplex PCR (mPCR) technology (Patent No. 7,999,092) that uses hundreds of VDJ specific primers in one reaction, semi-quantitatively and inclusively amplifying all of the expressed V(D)Js in B and T cells from a single sample.
Our primer kits are compatible with both Illumina and Roche 454 sequencing platforms. Each platform has unique properties that can be used to meet your project needs. Read more to find out which works best for you.
100 PER versus
Although we do offer primer kits for gDNA samples, we prefer to work with RNA as a starting template. There are a multitude of reasons why RNA is the preferred template, including being the rearranged gene product.
The V-C primer systems amplify the V through the C gene. Since the reverse primer is designed at an intron-exon junction, these primers are only compatible with RNA as a template. The V-J primers cover from the V through the J genes and are compatible with both RNA and gDNA.
Discover the difference between 100 paired-end read (100 PER) and 250 PER reagent systems by selecting the link above.
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